Bacteriocin typing of clinical isolates of Enterobacter cloacae
نویسندگان
چکیده
منابع مشابه
Purification and genetic determination of bacteriocin production in Enterobacter cloacae.
Enterobacter cloacae (strain DF13) was found to produce a bacteriocin which could be induced by mitomycin C. In the supernatant fluid of the induced culture phagelike particles were found. The bacteriocin was partially purified from induced cultures by ammonium sulfate precipitation and gel-filtration on Sephadex G-150. Ultraviolet-absorbing material was eluted from the Sephadex column in three...
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Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78...
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A typing system based on bacteriocin production and sensitivity for Streptococcus sanguis strains was devised. Bacteriocin producer strains were grown (37degrees C) anaerobically on brain heart infusion-yeast extract agar for 18 h. Bacteriocin indicator strains were suspended to molten brain heart infusion-yeast extract agar and then overlayed onto the producer strain. After an additional 18 to...
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The mechanisms of fluoroquinolone resistance in two isolates of Enterobacter cloacae, Ecl#1 and Ecl#2, from the same patient and with identical pulsed-field gel electrophoresis patterns, have been analysed. MICs of ciprofloxacin were 0.25 and 1 mg/L for Ecl#1 and Ecl#2, respectively. Ecl#2 was also more resistant to chloramphenicol and organic solvents. The quinolone resistance determining regi...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1982
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.16.5.885-889.1982